Enzyme Concentration On The Rate Of Reaction

INTRODUCTION: I am trying to investigate enzyme ingress and the rate of reaction. In this investigation, a proteolytic enzyme is used to modify state the breakdown of protein into peptides. Protein colloidal gel is the substrate used. As the reaction progress, the gelatin is broken down and become little viscous. This has effect on the time it takes for the gelatin to go with the displace in to the beaker. HYPOTHESIS: I predicted the higher the enzyme submergence, the less(prenominal) time it volition take for the gelatin to pass by dint of the funnel because the more than the proteolytic enzyme concentration, the faster the breakdown of the protein (gelatin) into peptides. EQUIPMENTS/ tool: Pipette, try pipage, trypsin, gelatin (protein), beaker, glass rod, clamp stand, funnel, founder quantify and ph buffer. subprogram: I used the pipette to touchstone 3 cm of pH buffer into a streamlet tube. The test tube was handled with c be to prevent it from breaki ng. I hence used some other pipette to time 7cm of 0% trypsin firmness into the same test tube containing the pH buffer. 75 gram of gelatin was weighed into a beaker. I then peppered the kind of pH buffer and the trypsin solution in the beaker containing 75 gram of gelatine. Using the glass rod, I carefully and softly stir the mixture for approximately 15 seconds, using the stop clock.

Using a clamp stand, I suspended the funnel over an empty beaker. I pour whole the mixture into the funnel after leaving it for 5 minutes and over again started the stop clock. I recorded all the time taken f or the gelatin to pass through the funnel. ! I repeated the performance for 1%, 3% and 5% of enzyme concentration varyingS: IN underage VARIABLE: Enzyme concentration (trypsin) was the main(a) variable because they are the changes that were directly caused by me. DEPENDENT VARIABLE: Rate of reaction (1/T) that is the time taken for the gelatine to pass through the funnel. This was the independent variable because it was the changes...If you want to see a full essay, enunciate it on our website: BestEssayCheap.com

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